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图解 | 单克隆免疫球蛋白相关性肾病(进阶版)

原标题:图解 | 单克隆免疫性球蛋白相关性肾病(进级版卡塔 尔(英语:State of Qatar)

定义

Circulating free DNA or Cell free DNA
(cfDNA):循环游离DNA只怕细胞游离DNA,释放到血浆中的分解的DNA片段。

图片 1

基脾性格

  • 长度
    图片 2

    Azad A A, Volik S V, Wyatt A W, et al. Androgen Receptor Gene
    Aberrations in Circulating Cell-Free DNA: Biomarkers of
    Therapeutic Resistance in Castration-Resistant Prostate
    Cancer.[J]. Journal of Urology, 2015, 194(3):704-704.

图片 3
在167bp左右(对应染色小体长度卡塔尔有三个峰,并且别的的峰有着10.5bp的周期波动,对应着核小体焦点DNA的螺距

Snyder M W, Kircher M, Hill A J, et al. Cell-free DNA comprises an in
vivo nucleosome footprint that informs its tissues-of-origin[J].
Cell, 2016, 164(1-2):57.

  • 生物学来源
  1. 凋亡和坏死

    Jahr S, Hentze H, Englisch S, Hardt D, Fackelmayer FO, et
    al. (2001) DNA fragments in the blood plasma of cancer patients:
    quantitations and evidence for their origin from apoptotic and
    necrotic cells Cancer Res 61:1659–65. pmid:11245480
    Giacona M B, Ruben G C, Iczkowski K A, et al. Cell-free DNA in
    human blood plasma: length measurements in patients with
    pancreatic cancer and healthy controls[J]. Pancreas, 1998,
    17(1):89.

  2. 移植物也许胎儿的游离DNA(参谋文献见下卡塔 尔(阿拉伯语:قطر‎

  3. 白手成家激活的DNA的放飞

    Stroun M, Lyautey J, Lederrey C, Olson-Sand A, Anker P (2001)
    About the possible origin and mechanism of circulating DNA
    apoptosis and active DNA release. Clin Chim Acta 313:139–42.
    pmid:11694251

  • 应用
  1. 胚胎的DNA会游离到孕妇的血液中,怀男孩的产妇血液能够检查实验到Y染色体,还可用于产前检查评定,21和18三体

    Lo YMD, Corbetta N, Chamberlain PF, Rai V, Sargent IL, Redman
    CWG,et al. Presence of fetal DNA in maternal plasma and serum.
    Lancet
    1997;350:485–7
    Bianchi DW, Parker RL, Wentworth J, Madankumar R, Saffer C, Das
    AF,et al. DNA sequencing versus standard prenatal aneuploidy
    screening.N Engl J Med 2014;370:799–808

  2. 器官移植免疫排斥爆发的细胞驾鹤归西,DNA会释放到血液中,由此用于器官移植状态的监督

    Beck J, Oellerich M, Schulz U, Schauerte V, Reinhard L, Fuchs U,
    et al.Donor-derived cell-free DNA is a novel universal biomarker
    for allograft rejection in solid organ transplantation. Transplant
    Proc 2015;47:2400–3

  3. cfDNA浓度与外伤、脱肛的损害程度有关

    Butt AN, Swaminathan R. Overview of circulating nucleic acids in
    plasma serum. Ann N Y Acad Sci 2008;1137:236–42

  4. 高浓度cfDNA也用于ICU呜呼哀哉的测度,也可用来预测脓毒症和脓毒性休克,无菌性炎症,心肌梗死和无影象学结果的脑颠簸病人患病情形。

    Volik S, Alcaide M, Morin R D, et al. Cell-free DNA (cfDNA):
    Clinical Significance and Utility in Cancer Shaped By Emerging
    Technologies[J]. Molecular Cancer Research Mcr, 2016:898-908.

  5. 癌症来源的cfDNA,能够发掘癌症相关的剧变、杂合子缺点和失误(LOH卡塔 尔(英语:State of Qatar)、基因扩大与扩张、癌病毒DNA、抑癌基因运营子区的超环甲苯化,因而得以兑今后无创条件下切磋肉瘤DNA

    Stroun M, Anker P, Maurice P, Lyautey J, Lederrey C, Beljanski M.
    Neoplastic characteristics of the DNA found in the plasma of
    cancer patients.
    Oncology 1989;46:318–22
    Chen XQ, Stroun M, Magnenat JL, Nicod LP, Kurt AM, Lyautey J, et
    al.Microsatellite alterations in plasma DNA of small cell lung
    cancer patients.
    Nat Med 1996;2:1033–5
    Chiang P-W, Beer DG, Wei W-L, Orringer MB, Kurnit DM. Detection
    oferbB-2 amplifications in tumors and sera from esophageal
    carcinoma
    patients. Clin Cancer Res 1999;5:1381–6
    Mutirangura A, Pornthanakasem W, Theamboonlers A, Sriuranpong
    V,Lertsanguansinchi P, Yenrudi S, et al. Epstein-Barr viral DNA in
    serum of
    patients with nasopharyngeal carcinoma. Clin Cancer Res
    1998;4:665–9
    Esteller M, Sanchez-Cespedes M, Rosell R, Sidransky D, Baylin SB,
    HermanJG. Detection of aberrant promoter hypermethylation of tumor
    suppressor
    genes in serum DNA from non-small cell lung cancer patients.
    Cancer Res1999;59:67–70.

  6. 核小体在TSS区是或不是攻下,影响cfDNA的深度,因而能够用来推论基因的表述境况

    Ulz P, Thallinger G G, Auer M, et al. Inferring expressed genes by
    whole-genome sequencing of plasma DNA.[J]. Nature Genetics,
    2016, 48(10):1273.

近日有战友已对该文实行总论翻译,个人感到各论部分也要命值得阅读与收藏,特整理为多少个表格,便于翻看。

(友情提醒:图片可点开放大,看得更明白卡塔尔国

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从前的原委参见

图解 | 单克隆免疫性球蛋白相关性肾病

仿效文献:

1、 Kourelis TV, Kumar SK, Gertz MA, Lacy
MQ,Buadi FK, Hayman SR, et al.:
Coexistentmultiplemyeloma or increased bone
marrowplasma cells define
equally high-risk populationsin
patients with immunoglobulinlight
chain amyloidosis. J Clin Oncol
31:4319–4324,
2013.

2、 Nasr SH, Valeri AM, Cornell LD, Fidler
ME,Sethi S, D’Agati
VD, et al.: Renal monoclonalimmunoglobulin deposition disease:
A reportof 64 patients from a single
institution.Clin J Am Soc Nephrol 7: 231–239,
2012.

3、 Lin J,Markowitz GS, Valeri AM, Kambham
N,Sherman WH, Appel GB, et al.: Renalmonoclonal immunoglobulin deposition
disease:The disease spectrum. J Am Soc
Nephrol12: 1482–1492, 2001.

4、 Bhutani G, Nasr SH, Said SM, Sethi
S,Fervenza FC,Morice WG, et
al.:Hematologiccharacteristics of proliferative
glomerulonephritideswith nonorganized monoclonalimmunoglobulin deposits. Mayo Clin
Proc90: 587–596,2015.

5、 Ramos-CasalsM, Stone JH,CidMC, Bosch
X:The cryoglobulinaemias. Lancet 379: 348–360,
2012.

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